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topflash and fopflash tcf reporter constructs  (Millipore)


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    Structured Review

    Millipore topflash and fopflash tcf reporter constructs
    Topflash And Fopflash Tcf Reporter Constructs, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/topflash+and+fopflash+tcf+reporter+constructs/tcf+reporter+constructs+topflash+and+fopflash/pm36255661-37-16-22
    Average 90 stars, based on 1 article reviews
    topflash and fopflash tcf reporter constructs - by Bioz Stars, 2026-10
    90/100 stars

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    Related Articles

    Luciferase:

    Article Title: CYP24A1 Involvement in Inflammatory Factor Regulation Occurs via the Wnt Signaling Pathway.
    Article Snippet: 1.2 Oligonucleotides The oligonucleotides for gene silencing were chemically synthesized by GenePharma (China) based on the sequence of CYP24A1 and are as follows: CYP24A1 small interfering RNA (siRNA) (siCYP24A1), 5′-CAAACAGUCUAAUGUGGAUTT-3′ (sense), 5′-AUCCACAUUAGACUGUUUGTT-3′ (antisense); siRNA negative control (siNC), 5′-UUCUCCGAACGUGUCACGUTT-3′ (sense), 5′-ACGUGACACGUUCGGAGAATT-3′ (antisense); GAPDH positive control, 5′-UGACCUCAACUACAUGGUUTT-3′ (sense), 5′-AACCAUGUAGUUGAGGUCATT -3′ (antisense). .. 1.3 Luciferase Assay To assay β-catenin/T-cell factor (TCF) activity, a luciferase reporter assay was performed using TOPflash and FOPflash TCF reporter constructs (Millipore, USA), which contained either the TCF/ lymphoid enhancer factor consensus sequence (TOPflash) or the mutant (FOPflash) TCF-binding sites, as described previously[13]. .. The PRL-TK plasmid was from Promega (USA), and DH5α was from Invitrogen (USA).

    Activity Assay:

    Article Title: CYP24A1 Involvement in Inflammatory Factor Regulation Occurs via the Wnt Signaling Pathway.
    Article Snippet: 1.2 Oligonucleotides The oligonucleotides for gene silencing were chemically synthesized by GenePharma (China) based on the sequence of CYP24A1 and are as follows: CYP24A1 small interfering RNA (siRNA) (siCYP24A1), 5′-CAAACAGUCUAAUGUGGAUTT-3′ (sense), 5′-AUCCACAUUAGACUGUUUGTT-3′ (antisense); siRNA negative control (siNC), 5′-UUCUCCGAACGUGUCACGUTT-3′ (sense), 5′-ACGUGACACGUUCGGAGAATT-3′ (antisense); GAPDH positive control, 5′-UGACCUCAACUACAUGGUUTT-3′ (sense), 5′-AACCAUGUAGUUGAGGUCATT -3′ (antisense). .. 1.3 Luciferase Assay To assay β-catenin/T-cell factor (TCF) activity, a luciferase reporter assay was performed using TOPflash and FOPflash TCF reporter constructs (Millipore, USA), which contained either the TCF/ lymphoid enhancer factor consensus sequence (TOPflash) or the mutant (FOPflash) TCF-binding sites, as described previously[13]. .. The PRL-TK plasmid was from Promega (USA), and DH5α was from Invitrogen (USA).

    Reporter Assay:

    Article Title: CYP24A1 Involvement in Inflammatory Factor Regulation Occurs via the Wnt Signaling Pathway.
    Article Snippet: 1.2 Oligonucleotides The oligonucleotides for gene silencing were chemically synthesized by GenePharma (China) based on the sequence of CYP24A1 and are as follows: CYP24A1 small interfering RNA (siRNA) (siCYP24A1), 5′-CAAACAGUCUAAUGUGGAUTT-3′ (sense), 5′-AUCCACAUUAGACUGUUUGTT-3′ (antisense); siRNA negative control (siNC), 5′-UUCUCCGAACGUGUCACGUTT-3′ (sense), 5′-ACGUGACACGUUCGGAGAATT-3′ (antisense); GAPDH positive control, 5′-UGACCUCAACUACAUGGUUTT-3′ (sense), 5′-AACCAUGUAGUUGAGGUCATT -3′ (antisense). .. 1.3 Luciferase Assay To assay β-catenin/T-cell factor (TCF) activity, a luciferase reporter assay was performed using TOPflash and FOPflash TCF reporter constructs (Millipore, USA), which contained either the TCF/ lymphoid enhancer factor consensus sequence (TOPflash) or the mutant (FOPflash) TCF-binding sites, as described previously[13]. .. The PRL-TK plasmid was from Promega (USA), and DH5α was from Invitrogen (USA).

    Construct:

    Article Title: CYP24A1 Involvement in Inflammatory Factor Regulation Occurs via the Wnt Signaling Pathway.
    Article Snippet: 1.2 Oligonucleotides The oligonucleotides for gene silencing were chemically synthesized by GenePharma (China) based on the sequence of CYP24A1 and are as follows: CYP24A1 small interfering RNA (siRNA) (siCYP24A1), 5′-CAAACAGUCUAAUGUGGAUTT-3′ (sense), 5′-AUCCACAUUAGACUGUUUGTT-3′ (antisense); siRNA negative control (siNC), 5′-UUCUCCGAACGUGUCACGUTT-3′ (sense), 5′-ACGUGACACGUUCGGAGAATT-3′ (antisense); GAPDH positive control, 5′-UGACCUCAACUACAUGGUUTT-3′ (sense), 5′-AACCAUGUAGUUGAGGUCATT -3′ (antisense). .. 1.3 Luciferase Assay To assay β-catenin/T-cell factor (TCF) activity, a luciferase reporter assay was performed using TOPflash and FOPflash TCF reporter constructs (Millipore, USA), which contained either the TCF/ lymphoid enhancer factor consensus sequence (TOPflash) or the mutant (FOPflash) TCF-binding sites, as described previously[13]. .. The PRL-TK plasmid was from Promega (USA), and DH5α was from Invitrogen (USA).

    Sequencing:

    Article Title: CYP24A1 Involvement in Inflammatory Factor Regulation Occurs via the Wnt Signaling Pathway.
    Article Snippet: 1.2 Oligonucleotides The oligonucleotides for gene silencing were chemically synthesized by GenePharma (China) based on the sequence of CYP24A1 and are as follows: CYP24A1 small interfering RNA (siRNA) (siCYP24A1), 5′-CAAACAGUCUAAUGUGGAUTT-3′ (sense), 5′-AUCCACAUUAGACUGUUUGTT-3′ (antisense); siRNA negative control (siNC), 5′-UUCUCCGAACGUGUCACGUTT-3′ (sense), 5′-ACGUGACACGUUCGGAGAATT-3′ (antisense); GAPDH positive control, 5′-UGACCUCAACUACAUGGUUTT-3′ (sense), 5′-AACCAUGUAGUUGAGGUCATT -3′ (antisense). .. 1.3 Luciferase Assay To assay β-catenin/T-cell factor (TCF) activity, a luciferase reporter assay was performed using TOPflash and FOPflash TCF reporter constructs (Millipore, USA), which contained either the TCF/ lymphoid enhancer factor consensus sequence (TOPflash) or the mutant (FOPflash) TCF-binding sites, as described previously[13]. .. The PRL-TK plasmid was from Promega (USA), and DH5α was from Invitrogen (USA).

    Mutagenesis:

    Article Title: CYP24A1 Involvement in Inflammatory Factor Regulation Occurs via the Wnt Signaling Pathway.
    Article Snippet: 1.2 Oligonucleotides The oligonucleotides for gene silencing were chemically synthesized by GenePharma (China) based on the sequence of CYP24A1 and are as follows: CYP24A1 small interfering RNA (siRNA) (siCYP24A1), 5′-CAAACAGUCUAAUGUGGAUTT-3′ (sense), 5′-AUCCACAUUAGACUGUUUGTT-3′ (antisense); siRNA negative control (siNC), 5′-UUCUCCGAACGUGUCACGUTT-3′ (sense), 5′-ACGUGACACGUUCGGAGAATT-3′ (antisense); GAPDH positive control, 5′-UGACCUCAACUACAUGGUUTT-3′ (sense), 5′-AACCAUGUAGUUGAGGUCATT -3′ (antisense). .. 1.3 Luciferase Assay To assay β-catenin/T-cell factor (TCF) activity, a luciferase reporter assay was performed using TOPflash and FOPflash TCF reporter constructs (Millipore, USA), which contained either the TCF/ lymphoid enhancer factor consensus sequence (TOPflash) or the mutant (FOPflash) TCF-binding sites, as described previously[13]. .. The PRL-TK plasmid was from Promega (USA), and DH5α was from Invitrogen (USA).



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